IAC-USNC Methods in Neuroscience- Single Channel Recording

Sylgard Firepolish 1 Firepolish 2 Check Pipettes Recording SC Home

Below are photos of some of the equipment used in single channel recording. Of course not all experiments involve a multi-line perfusion system, such as that shown below.

Some equipment used to record single channel currents
The microscope is enclosed in a Faraday cage to reduce the influence of stray electrical noise. In addition, the scope sits on an air table to reduce noise due to vibration.	Voltage paradigms are delivered and data is recorded with the help of a computer and a patch clamp amplifier.	Here's an example of what some data might look like on the computer monitor.
A view of the microscope stage.	A more close-up view showing the two tubes (green arrows) that perfuse the patch with control bath solution and the tube (pink arrow) from which the test solutions flow.
In this experiment, 12 different test solutions are available.	A more close-up view of the perfusion system.	A small computer, different than the main computer which controls the experiment, is used to control the flow of the 12 test solutions. This computer is controlled by the main computer.