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Methods in
Neuroscience Drosophila Neuromuscular Junction - Dissection p2 |
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Below are photos of some of the key steps in dissecting the drosophila larva to expose
muscle #6 and its two innervating neurons. |
| Dissecting the larva
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![]() The larva is pinned anteriorly and posteriorly under the dissecting scope. |
![]() This is the view under the dissecting scope. |
![]() A cut is made across the posterior end. |
![]() After the cut is made the preparation looks like this. |
![]() An incision is then made along the midline... |
![]() from the posterior... |
![]() all the way to the anterior end. |
![]() This is what it looks like anteriorly when the midline cut is completed. |
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![]() Beginning at the posterior end, the forceps are used to gently pull away and remove the ___ as the scissors cuts the attaching tissues beneath. |
![]() this continues anteriorly |
![]() ... |
![]() ... |
![]() until all is removed. |
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![]() The preparation looks like this now. |
The anterior end looks like this up close. |
![]() When the dissection is compete. |
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